منابع مشابه
Thermolysin-catalyzed peptide bond synthesis.
The rates of the thermolysin-catalyzed synthesis of peptides have been determined by means of HPLC. In the condensation of various N-substituted amino acids and peptides with L-leucinanilide, the enzyme exhibits preference for a hydrophobic L-amino acid as the donor of the carbonyl group of the newly formed bond. The presence of another hydrophobic amino acid residue adjacent to the carbonyl-gr...
متن کاملMechanism of peptide bond synthesis on the ribosome.
With the emergence of atomic-resolution crystal structures of bacterial ribosomal subunits, major advances in eliciting structure-function relationships of the translation process are underway. Nevertheless, the detailed mechanism of peptide bond synthesis that occurs on the large ribosomal subunit remains unknown. Separate x-ray structures of aminoacyl-tRNA and peptidyl-tRNA analogues bound to...
متن کاملThe structural basis of ribosome activity in peptide bond synthesis.
Using the atomic structures of the large ribosomal subunit from Haloarcula marismortui and its complexes with two substrate analogs, we establish that the ribosome is a ribozyme and address the catalytic properties of its all-RNA active site. Both substrate analogs are contacted exclusively by conserved ribosomal RNA (rRNA) residues from domain V of 23S rRNA; there are no protein side-chain ato...
متن کاملPepsin and Thermolysin as Catalysts of Peptide Bond Synthesis
The early studies on proteinase-catalyzed peptide bond synthesis are summarized as background for a report on some recent work in our laboratory on pepsin and thermolysin. The primary and secondary specificity of these two enzymes have been found to be suitable for the preparative synthesis of oligopeptides by the condensation of two hydrophobic L-amino acid residues. Kinetic data are presented...
متن کاملPeptide bond vibrational coupling.
Neutral trialanine (Ala3), which is geometrically constrained to have its peptide bond at Phi and Psi angles of alpha-helix and PPII-like conformers, are studied at the B3LYP/6-31+G(d,p) level of theory to examine vibrational interactions between adjacent peptide units. Delocalization of the amide I, amide II, and amide III3 vibrations are analyzed by calculating their potential energy distribu...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1947
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)35067-6